Eastern Michigan University
DNA Profile Database
This site allows for the submission and analysis of DNA profiles generated based on Alu presence/absence variants. For more information on the technique refer to Kass (2003) "Generation of human DNA profiles by Alu-based multiplex polymerase chain reaction" Analytical Biochemistry 31:146-149, and Kass (2007) "Simple and rapid generation of complex DNA profiles for the undergraduate laboratory" The American Biology Teacher 69:163-168.
I. Profile Incorporation
This site will allow you to anonymously incorporate your DNA profile generated by PCR amplification of presence/absence Alu variants.
The instructor will first register, then register the class.
You can register and choose the class you belong to. The Submit Profile link will allow you to add your profile to the database.
The View Profile link allows you to see your profile.
II. Analysis
This site will allow for genotype analyses from all submitted profiles including comparisons within and between populations.
A) Comprehensive Profiles
The comprehensive profiles compiles all individual information submitted to this database. Frequencies of all profiles are calculated and provided in the Profile Listings link. By viewing the frequencies of different profiles the user of this technique is provided information helpful for class forensic studies. The Genotype Listings link allows for analysis of the individual loci from all profiles submitted to this database.
B) Class Profiles
The View Classes and the Population Data links permit viewing genotype and allele frequencies within the class, as well as providing information of the level of heterozygosity and determining if this "population" is in Hardy-Weinberg equilibrium.
C) Class and Population Comparisons
The Population Comparisons link allows for selection of classes to compare.
Information is provided regarding each class and combined information is generated.
The chi-square calculation compares genotype distribution between the two populations.
A p-value less than 5% indicates real genetic differences between the populations.
The genetic drift calculation, based on the model of Sewell Wright uses heterozygosity
values to determine the number of generations these populations have been allowed to grow apart.
The Fst unbiased genetic distance calculation quantifies the proportion of genetic variation that lies between
subpopulations within the total population.
Its value can be considered inversely proportional to gene flow, indicative of the length of time the two populations
have been evolving separately.
A general guideline for Fst value interpretation can be as follows:
0 - 0.05 ~ little genetic differentiation
0.05 - 0.15 ~ moderate genetic differentiation
0.15 - 0.25 ~ great genetic differentiation
0.25 ~ very high genetic differentiation
0.05 - 0.15 ~ moderate genetic differentiation
0.15 - 0.25 ~ great genetic differentiation
0.25 ~ very high genetic differentiation
We hope you find this site useful and rewarding.
Contact Information (Comments, suggestions, questions, etc.):
Dr. David Kass
Department of Biology
316 Mark Jefferson
Eastern Michigan University
Ypsilanti, MI 48197
dkass@emich.edu
http://www.emich.edu/public/biology/kass.html
Webmaster: Robert LaRoe
Department of Biology
316 Mark Jefferson
Eastern Michigan University
Ypsilanti, MI 48197
dkass@emich.edu
http://www.emich.edu/public/biology/kass.html
Webmaster: Robert LaRoe